Endringer i matinntak og metabolisme i ny dyremodell for nattskiftarbeid

I denne studien har samme dyremodell blitt brukt for å undersøke effektene av simulert nattskiftarbeid på metabolske mål og døgnrytme. Det ble undersøkt om endringer i måltidsrytme kunne forklares av endringer i uttrykk av metabolske gener i lever relatert til energisparing og insulinmetabolisme. Studien er utført av forskere fra Universitetet i Bergen og Nederland.

 

Studien er publisert i Nutrients


Shift in Food Intake and Changes in Metabolic Regulation and Gene Expression during Simulated Night-Shift Work: A Rat Model


Andrea Rørvik Marti, Peter Meerlo, Janne Grønli, Sjoerd Johan van Hasselt, Jelena Mrdalj, Ståle Pallesen, Torhild Thue Pedersen, Tone Elise Gjøtterud Henriksen, Silje Skrede


Night-shift work is linked to a shift in food intake toward the normal sleeping period, and to metabolic disturbance. We applied a rat model of night-shift work to assess the immediate effects of such a shift in food intake on metabolism. Male Wistar rats were subjected to 8 h of forced activity during their rest (ZT2-10) or active (ZT14-22) phase. Food intake, body weight, and body temperature were monitored across four work days and eight recovery days. Food intake gradually shifted toward rest-work hours, stabilizing on work day three. A subgroup of animals was euthanized after the third work session for analysis of metabolic gene expression in the liver by real-time polymerase chain reaction (PCR). Results show that work in the rest phase shifted food intake to rest-work hours. Moreover, liver genes related to energy storage and insulin metabolism were upregulated, and genes related to energy breakdown were downregulated compared to non-working time-matched controls. Both working groups lost weight during the protocol and regained weight during recovery, but animals that worked in the rest phase did not fully recover, even after eight days of recovery. In conclusion, three to four days of work in the rest phase is sufficient to induce disruption of several metabolic parameters, which requires more than eight days for full recovery.